Dyslipidemia is unhealthy levels of one or more kinds of lipids in blood that are high density lipoprotein (HDL), low density lipoprotein (LDL), triglycerides. LDL cholesterol is considered as the ‘bad’ types of cholesterol, HDL as the ‘good’ type of cholesterol, and triglycerides are stored in fat cells, it comes from the calories that we eat. It is major cause for various lifestyle disorders. Which leads the risk factor for atherosclerotic coronary heart disease. Hence as a preventive purpose and with logical approach to dietary intervention with pranayam, this study has conducted to assess the effect of flaxseeds powder as diet supplement with pranayam on serum blood lipid profile of selected dyslipidemia subjects. The study was conducted at D.Y. Patil Ayurvedic hospital Nerul, Navi-Mumbai. 40 Subjects were enrolled according to inclusive and exclusive criteria. Statistical analysis was done with help of paired T test and Mann Whitney which shows (P<0.005) i.e, significant reduction only in serum triglyceride, LDL-cholesterol in group A which was supplemented with roasted Flaxseed powder of 30 gm for 8 weeks with pranayam. On the other hand, non- significant result as (P>0.005) were observed in all the parameter of group B subjects to which only flaxseed powder was incorporated as diet supplement, Thus the study suggests that roasted Flaxseeds powder incorporated as diet supplement with pranayam would be considered as effective agent for lipid lowering purposes if used for longer duration of time.
Adsorbent for removal of Cd (II) was prepared by grinding sweet lime tree leaves. The removal of toxic heavy metal like Cd (II) was studied using adsorption technique. In the present study the adsorption of toxic heavy metal from industrial wastewaters on to sweet lime (Citrus limetta) tree leaf powder (CLTLP) was studied. The percent adsorption varies with adsorbent dose, pH and contact time. Adsorption model of CLTLP well fitted in Langmuir as well as in Freundlich model. The present investigation shows that CLTLP is an effective adsorbent for the removal of Cd (II) from aqueous solutions.
The electronic structure of 3-(2-methylphenylaminothiazol-5-oyl) pyridine was analysed theoretically using B3LYB/6-31G method. The calculations exhibit that the compound non-planar, as observed from the dihedral angles. The electronic spectra of the studied compound are recorded in UV-VIS region, in ethanol as solvent. The identified electronic transitions are facilitated via density functional theory (DFT) computations. Electronic configurations according to every excited state are recognized and the suitable MOs are characterized. The range of delocalization and intermolecular charge transfer was evaluated and considered in terms of natural bond orbital (NBO) analysis. The Mulliken atomic charge describe the charge distribution of atoms. The calculated HOMO-LUMO energy gap unveils that charge transfer arise within the molecule.
S. Mahil Rani*, S. Viola Rose, T. F. Abbs Fen Reji.
Hydrogen peroxide (H2O2) is an essential compound in food, pharmaceutical, clinical, industrial and environmental analysis. H2O2 has been widely used for preservation of food products due to its bactericidal properties. However, excess of H2O2 can be deleterious on the nutritional value of food. In the present research work a simple and sensitive spectrophotometric assay was developed for the quantification of H2O2 in different samples. This method involves the reaction of Iso-nicotinic acid hydrazide (INH) and pyrocatechol (PC) as co-substrates in presence peroxidase enzyme and H2O2 to form a colored product, which has strong absorbance at λmax = 520 nm. The effects of different parameters on the sensitivity of the proposed assay were studied. The Michaelis-Menten constant and catalytic efficiency were also calculated. Some of the important advantages of the proposed method are, use of inexpensive reagents, simple operation conditions, rapid analysis, wide linear range, high sensitivity, free from interfering substances and precision comparable to the reference Gilliland method. The proposed method was used for analysis of different samples.
Uddanam Region of srikakulam district, a North Coastal Andhra Pradesh state is gifted with full of lush greenery and vegetation stretched along the coastal belt of Bay of Bengal. Uddanam region suffers with high prevalence of chronic kidney disease (CKD) presenting clinically or sub clinically ultimately leading to high mortality and morbidity with RRT and other types of diseases. Ground water samples were collected from different areas such as Kopasakudi, Rajapurarn, Kaviti, Khojiiria and Palasa control of uddanam region were analyzed for heavy metals such as Hg, Fe, Mn, Se, Cu, Zn and phenolic compounds by using as per the standard methods for the examination of water and wastewater by APHA, WEF and AWWA. Among the seven heavy metals detected in the soils of the study area, only Hg, Se and phenolic compounds such as C6H5OH are within the permissible limits. The increased levels of Fe, Mn, Cu and Zn in the study area are a major concern for the agricultural and land practices. Based on test results, copper shows the least concentration among the heavy metals and while iron shows the highest concentration in the soil samples. However these elements need continuous monitoring in this uddanum region which may enter the food chain and maybe hazardous to human health.
Introduction: The present study was undertaken to examine the effect of beverages such as apple juice, orange juice, pineapple juice, watermelon juice, lemon juice etc. and their interaction on common OTC drug like Paracetamol which is analgesic and antipyretic, usually in the form of an immediate release tablet formulations. Therapeutic effects in terms of the speed and intensity of the analgesic effect is dependent on speed of liberation from formulation. Paracetamol can interact with the food or beverages, which may reduce the drug release, drug absorption in the body and may also lead to an unwanted reaction. Methods: In vitro Dissolution study was done on these beverages, using phosphate buffer as dissolution media of pH 5.8 with USP Type II apparatus. The samples from dissolution were analyzed under HPLC and UV method. Results: The dissolution of drug was found to be less in all of these beverages than in drinking water. It was observed that, when paracetamol is taken with drinking water, maximum drug dissolution takes place i.e. 97.03% and 97.92% by UV spectroscopy and HPLC method respectively as compared to other beverages. The minimum amount of drug release was observed in apple juice i.e. 16.90% and 16.93% by UV spectroscopy and HPLC method respectively when compared with orange, pineapple, watermelon, and lemon juice. The maximum amount of drug release is observed in lemon juice i.e. 39.79% and 43.14% by UV spectroscopy and HPLC method respectively as compared to remaining four juices. Conclusion: To get better therapeutic effects without any side effects the drug should be taken with glass of drinking water and avoid taking medicines with beverages. Medicines should not be taken with the meal to avoid any food-drug interactions. The physicians and pharmacists recognize that some foods and drugs, when taken simultaneously, can alter the body's ability to utilize a particular food or drug.
The aim of the work was to investigate the phytoconstituents, antioxidant property and hepatoprotective activity of Pavetta indica herb. The aerial parts were dried, powdered and extracted by using a soxhlet extractor. Extracts were screened for phytoconstituents and total antioxidant property was evaluated by phosphomolybdenum method. In vitro hepatoprotective activity was determined by MTT assay method and in vivo study was performed by CCl4 induced hepatotoxicity in male wistar albino rats. The results reveal that the methanolic extract have a potential antioxidant and hepatoprotective activity.
A series of novel aromatic poly and copoly (amide-imide)s (PAIs) are prepared from two dicarboxylic acid anhydride (PMDA, BTDA) a and synthesized diamines. Synthesis of a new diamine containing thiazolidine unit N, N’-bis-[3-(2-amino-thiazol-4-yl)-phenyl] isophthalamide (ATPIPA) was synthesized by reaction of isophthaloyl chloride with m/p-aminoacetophenone followed by reaction with iodine, thiourea and characterized by different spectroscopic methods. Diamines and all polymers are characterized by FT-IR, NMR spectroscopic techniques. The polymers obtained have good thermal stability and glass transition temperature (Tg) in the range of 170-185oC. The resulting polymer exhibit inherent viscosity in the range of 0.2 to 0.37 dL/g. These polymers are found to be soluble in aprotic polar solvents such as NMP, DMSO, DMF and DMAc. Wide angle X-ray diffraction revealed that these polymers are predominantly semicrysatlline to amorphous in nature.
Cancer is distributed over a large area whereas it is threat to humanity. In prevention and treatment of cancer, plants play an important role. The scientific name of Nagalingam is Couroupita guianensis Linn (Family: Lecythidaceae) a tree found throughout the plains of India. It inhibits the growth of microorganisms and show larvicidal activity against vectors. Among female fatal breed of cancers is breast Cancer. It is the cancer originating from intramural lining of milk ducts a tissue of breast, particularly which impart the ducts with milk. Hsp90 is an abundant protein in mammalian cells. Hsp90 plays a major role in breast neoplasia, which is aboundant in breat tissue. Methodology: The method involved the ligand preparation, protein preparation, grid generation and docking against the target using GLIDE - Maestro 2018. The sixteen active constituents present were the ligands. The target protein is 3TUH downloaded from protein data bank. Results and discussion: The docking score were Isatin -5.48, Indigo -5.29, Coup2 -5.23, Indirubin-5.25, Calotronaphthalene -5.06, Coup -4.99, Alpha Amyrin -4.94, Nerol-4.75, Betasitosterol -4.74, Campesterol-4.68, Eugenol -4.61, Tryptanthrin -4.31, Benzyl Alcohol -4.10, Betaamyrin -3.07 and Farnesol -3.06. The protein is validated before docking with ligand 3TUH_TUH_A_1 with score of -9.53 and standard dauxorubicin with docking value of - 6.51. Conclusion: Among the docked compound, isatin had good docking score comparable to standard. It had shown interaction with the ASN 51 like standard and interacted with LYS 58 like the validated compound. In future scope of work the isatin can be screened against breast cancer.
Gopinathan Narasimhan*, Yerramilli Lalitha Sravya, Chitra Krishnan, D. Chamundeeswari.
Raloxifene, a second-generation selective estrogen receptor modulator uses to prevent breast cancer and osteoporosis in postmenopausal women is administered orally in the form of tablet. The absolute bioavailability of the drug is only 2% because of extensive hepatic first-pass metabolism. To tackle this problem, co-polymeric pluronic conjugate micelles, which are amphiphilic copolymers, were designed and evaluated in this study. These highly stable micelles with raloxifene the core were formulated by thin film hydration method using various types and combinations of non-ionic surfactant (span60), co-polymeric surfactants (pluronic L64 pluronic P85), and charge inducing agent (DCP). The prepared mixed raloxifene niosomes were characterized for particle size, PDI, zeta potential. FTIR and DSC studies revealed that there was no interaction between the drug and Excipients. SEM studies showed nearly spherical shaped vesicles. F5 showed highest % drug content more than 98% entrapment efficiency 94.64±0.48% and % of in-vitro release 99.32±0.06% was obtained at 24th hour. In-vitro kinetics release rate for the all formulations followed zero order mechanism. The Optimized F6 formulation was subjected to three months for stability studies at temperature 5°C ± 3°C showed that there is not much variation in the % drug release.