A highly efficient methodology has been described for Synthesis of derivatives 7, 7-dimethyl-4-phenyl-2-thioxo-2, 3, 4, 6, 7, 8-hexahydro-1H-quinazolin-5-one derivatives. These derivatives can be synthesized from dime done and substituted aromatic aldehydes with thiourea in the presence of trifluoro acetic acid catalyst with moderate to excellent yields under reflux through condensation followed by cyclization to give desired products. All structures of products were confirmed by ¹H NMR and ¹³C NMR spectroscopy. In addition to evaluated of the bioevaluation.

In present study, the synthesis of novel series of 2-phenyl-5-(pyridin-3-yl)-1, 3, 4-oxadiazole. These derivatives were obtained fromNicotinoyl hydrazide with substituted aromatic aldehydes in gridding process. The Nicotinoyl hydrazide can be synthesized by Nicotinoyl chloride with hydrazine hydrate in ethanol at reflux thionyl chloride in presence of DCM at 5-10^oC. The chemical structures of newly synthesized compounds were confirmed by IR, 1HNMR, 13CNMR, Mass spectral techniques and nitrogen (%) analyses. All these synthesized compounds were investigated for their antibacterial activities against bacterial strains i.e. S.aureus, B. subtilis, E.coli and P. aeruginosa.

A Heterocycles molecule possesses benzimidazoles scaffold are of great important and interest because they represent an important class of numerous synthetic compounds which have beneficial biological properties. In this article, the synthesis of new N-substituted benzimidazole derivatives 5a-5f described. These compounds were prepared by first designing the N-substituted benzimidazoles (5a-f). The derivative 3a was obtained from the condensation reaction between O-Phenylene diamine and p- chlorobenaldehyde in presence of methane sulfonic acid as a catalyst. All the compounds were evaluated by advanced spectroscopic data (1H NMR, 13C NMR and LCMS) and the structural determination of the novel derivations was calculated by elemental analysis. In the present study, ten hybridized acridine derivatives were synthesized via cyclo condensation and evaluated for their in vitro antifungal Activity antimicrobial activity.

During this study, a reverse phase gel permeation chromatographic method to quantitate the amount of protease enzyme released from Serratiopeptidase enteric coated tablets, was developed. An isocratic mobile phase in combination with gel column was finally optimized for the study. Serratiopeptidase is a Proteolytic enzyme produced by purification from the culture of non-pathogenic enterobacterium Serratia sp. E-15. It hydrolyses the protein Casein into its constituent amino acids at a selected temperature and pH. It is used to reduce pain and swelling related to conditions like back pain, arthritis, tension headaches, migraine headaches etc. the tablet is intended for targeted delivery in intestine and the drug release had been monitored in pH 6.8 phosphate buffer media. The GPC technique had been applied to separate enzyme from excipients. The result showed that the chromatography is suitable to separate and quantitate the amount of drug released from tablet in presence of other excipient and coating materials.

The extensive applications of biosurfactant due to their diverse properties like specificity, low toxicity compared to chemical surfactants and stability in wide range of environmental conditions has increased its importance. In the present study, solubilization of anthracene by the biosurfactant produced by Bacillus thuringiensis and Proteus vulgaris was found to be high when compared to the chemical surfactant - SDS added with anthracene. Statistical analysis was used to confirm the studies performed for 24 hours in the presence of biosurfactant produced by Bacillus thuringiensis and Proteus vulgaris. Results of GC-MS analysis revealed that the solubilization of diesel by Bacillus thuringiensis and Proteus vulgaris.

Zn-Mn-Al₂O₃ pervoskite was doped in polypyrrole solution in accordance to weight percentage composition of 5%, 10%, 15%, 20%, and 25% by a two-step method via wet impregnation method at ambient temperature resulted in Zn-Mn-Al₂O₃ doped polypyrrole nano composite thin films are conductive and porous. The Zn-Mn-Al₂O₃ /PPy was being characterized using various methods. Thin films generated, comprising of two-probe electrical resistivity, scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and X-ray diffraction. The structural formation of Zn-Mn-Al₂O₃ /polypyrrole was determined using FTIR, XRD, and SEM investigations. All thin films have shown decrease in electrical resistivity as concentration of Zn-Mn-Al₂O₃ increases as dopant with polypyrrole, which indicates higher conductivity of thin films with Zn-Mn-Al₂O₃ as a dopant. Surface morphology of Zn-Mn-Al₂O₃/polypyrrole materials has been discovered substantial influence on the thermal stability and the ability to detect gases. All the thin films were studied for the H₂S gas sensing behavior. The antimicrobial and antifungal activities of the investigated PPy and its PPy/Zn-Mn-Al₂O₃ nanocomposite materials using the cup plate technique at various doses in DMSO solvent using the minimum inhibitory concentration (MIC) method. The antimicrobial study indicates that its PPy/Zn-Mn-Al₂O₃ (20%) and PPy/Zn-Mn-Al₂O₃ (25%) have high microbial activity, whereas PPy has moderate activity as compared to standard.

Co(II), Ni(II) and Cu(II) metal complexes with 3-(2-hydroxyphenyl)-1-phenyl-1H-pyrazole-4-carbaldehyde (HL¹), 3-(5- chloro-2-hydroxyphenyl)-1-phenyl-1H-pyrazole-4-carbaldehyde (HL²) and 3-(5-bromo-2-hydroxyphenyl)-1-phenyl-1H-pyrazole-4-carbaldehyde (HL³) ligands derived from reaction of 2-hydroxyacetophenonephenyl hydrazonewith POCl₃have been prepared. Elemental analysis, conductance measurements, magnetic moment, infrared, NMR, ESR and electronic spectral studies have all been used to characterise the complexes. The complexes were discovered to exhibit ML₂, square planar shape and stoichiometry (1:2). The stability constants of Co(II), Ni(II) and Cu(II) metal complexes have been examined spectrophotometrically at an absorption maximum of 415nm, 340nm and 610nm at various temperatures.

Piper wightiia well-known medicinal plant of Piperaceae family is used by many tribal groups to treat inflammation in India. In the study, different extracts such as petroleum ether, chloroform, ethyl acetate, ethanol and hot water of Piper wightiileaves are evaluated for its antioxidant, antibacterial and anti-inflammatory activity and phyto constituents are profiled using Gas Chromatography – Mass Spectrometry (GC-MS). Among various extracts, leaf ethanol extracts showed the maximum amount of phenolics (336.84mg GAE/g extract), tannin (331.53mg GAE/g extract) and flavonoids ethyl acetate (174.88mg RE/g extract) content. It also revealed the presence of highest antioxidant property by estimating DPPH% (IC₅₀: 21.03μg/mL), ABTS+ (64166.67μM TE/g extract), Superoxide (44.84%) radical scavenging activity and Phosphomolybdenum ethyl acetate (177.25mg AAE/g). Moreover, ethanol extract exhibited good antibacterial activity against Klebsiella pneumonia(22.03mm), Escherichia coli(22.35mm), Salmonella typhimurium(25.23mm) Bacillus subtilis(22.3mm), Staphylococcus aureus(20.37mm), at 20mg/ml concentration. The ethanol extract showed high degree of inhibition (63.69%) in anti-inflammatory assay. GC-MS depicted the presence E, E, Z-1, 3, 12-Nonadecatriene-5, 14-diol component primarily in leaf ethanol extract. Piper wightiileaf extracts have a tremendous amount of antioxidant potential, making them a good source of natural antioxidant supplements for food to protect against oxidative stress-related diseases, including inflammation.

Helminthic infections are one of the serious diseases affecting humans and animals. The aim of this study to evaluate anthelminthic and antibacterial efficiency of methanolic extract of Cucurbita maxima (pumpkin leaves) against Indian adult earthwom Pheretima posthuma. Pumpkin leaf extraction done by Accelerated solvent extraction and screened for the presence of phytochemicals both qualitatively and by GC-MS analysis. These procedure methods revealed the presence of flavanoids, terpenoids, saponins, tannins, phenols, carbohydrates, glucosides and sterols. Anthelminthic activity results were expressed regards to paralysis and death. Three worms were released into petridishes containing solutions of different concentrations of extracts (20, 40, 60mg/ml), saline was taken as control and albendazole as standard. Antibacterial activity done by agar well diffusion method using clinical specimens. Antioxidant activity of leaf extract evaluated by DPPH free radical scavenging assay.

Objective: Antacids are popularly used throughout the world to address Gastric ulcer problems. In the present study aqueous extract of fennel seeds (Foeniculum vulgare), fenugreek seeds (Trigonella foenum-graecum) and 1:1 solution of these seeds are estimated of their antacid and antiflatulence activity. Methods: The pH of aqueous extracts and their neutralizing properties on artificial gastric acids were determined and compared with water and among themselves. An artificial stomach was used to evaluate the duration of consistent neutralization effect on artificial gastric acids. The anti-flatulent activity was tested by creating foam with sodium laurethsulphate, and volume reduction of foam was calculated post addition of sample solutions. Results: The results showed that aqueous extract of both fennel and fenugreek seeds has considerable antacid and anti-flatulent. Compared to the water group, aqueous extract of seeds was found to have significant (P<0.05). Gastric acid neutralizing effects. The duration for consistent neutralization of fennel aqueous extract was significantly longer than the other sample solution. Moreover, these samples exhibited distinct anti-flatulent effects in in vitro model scoring marginally higher value for fenugreek. Conclusion: Both anti-acidic and anti-flatulence activity recorded by fennel extract in water and also by fenugreek highly corroborate with popular uses of these spices as ethnomedicine and can be recommend in different herbal formulation to cure peptic ulcer problems.