Plants produce a diverse range of secondary metabolites, making them an excellent source of a variety of medicines. The continued and widespread use of medicinal plants around the world has raised concerns about their safety, efficacy and quality. As a result, proper knowledge of phytochemical is required. Plant bio-active constituents are relevant because this information will be useful in the synthesis of new drug formulations. The current investigation’s primary goal was to investigate the qualitative preliminary findings. Melochia corchorifoliaand Ludwigia perennis analysis, both plants are well-known medicinal plants because of their diverse pharmaceutical properties. The investigation of its qualitative phytochemical analysis for stem, root, leaf, fruit of Melochia corchorifolia and stem, bark, leaf, root for Ludwigia perennis is critical for the discovery of several other compounds. To obtain this result, five different solvents were used; water, ethanol, methanol, carbinol extracts of different plant parts. These extracts were used for qualitative preliminary phytochemical analysis using standard chemical tests. Data show the presence of alkaloids, flavonoids, steroids, terpenes, tannins, saponins, phenolic compounds, cardiac glycosides and reducing sugars. The ethanol extract of Melochia corchorifolia and chloroform extract of Ludwigia perennis contained the majority of the phytochemical components. Because of the active compounds of Melochia corchorifolia highly soluble in ethanol and active compounds of Ludwigia perennis highly soluble in chloroform. The presence of these secondary bioactive phytochemicals indicates the significance of these medicinal plants as an effective sources for the therapeutic agents.
Syamkumar T S*, Geethalakshmi Sundararaman, Anu Augustine.
Novel benzothiazepines were designed and subjected for molecular docking study and the compounds possess significant interaction with the targeted enzyme topoisomerase II. Among them compound BD3and BD6 (-7.9kcal/mol) showed similar docking compared to Adriamycin (-12.76kcal/mol). Based on the experimental results, among all the compounds synthesized, compound BD1 substituted with acetaldehyde showed good inhibition (127.02µg/mL). The in-vitro cytotoxic evaluation was carried out for the synthesised compounds and the results were found that the synthesized compounds are relatively non-toxic at 100µg concentration in tested cell line. Among the tested compounds, compound BD3 substituted with methyl benzene group were found to have IC50 values 46.4023µg/mL against MCF – 7 cancer cell lines. Compound BD6 substituted with chloro-benzene were found to have IC50 values 95.8213µg/mL against MCF7 cancer cell lines. Based on the study the compound BD1, BD3 and BD6 have emerged to be the most active compounds.
Plants rely on a class of proteins known as transcription factors (TFs) to regulate their physiological and biochemical functions. Among the many different types of TFs, MYB family proteins are the largest and play a crucial role in regulating plant growth, development, and stress responses. MYB TFs have been extensively studied in numerous dicot and monocot plant species, but very little is known about them in the complex polyploidy genome of sugarcane. In order to better understand MYB genes in sugarcane, this study utilized biocomputational analysis to compare closely related Sorghum bicolor plants. The MYB genes in Saccharum officinarum Co86032 were then identified using the Primer blast tool and primers were designed specifically for these genes. The primers were used in PCR to amplify the MYB genes from both the leaf and stem tissues of the sugarcane plant. The resulting amplicons were sequenced to verify the presence of MYB genes. The open reading frame (ORF) of the sequenced amplicons was then checked using an ORF finder, and the resulting ORFs were analyzed for the presence of MYB gene domains using ScanProsite. The MYB protein sequences were further analyzed to identify nuclear export signals (NES) using Loc NES. The MYB transcription factor super family plays a critical role in plant growth and defense mechanisms. Identifying and analyzing the MYB genes in sugarcane is therefore of great importance for genetic crop improvement, particularly regarding abiotic stress. By better understanding the MYB domains in the upstream region, it may be possible to induce the over-expression of MYB genes, thereby enhancing sugarcane's resistance to abiotic stress. This study provides valuable insights into the MYB family of proteins in sugarcane, which could have far-reaching implications for the development of more resilient crops. By identifying and understanding the genes responsible for regulating plant growth and stress responses, we can help to ensure that crops are better equipped to handle the challenges posed by the environment.
M. Babu*, Geethalakshmi Sundararaman, Anu Augustine, T. S. Syamkumar.