The study was undertaken to investigate antioxidant activity of annona squamosa linn using Ferric reducing assay on different fractions. Ethyl acetate fractionof Annona squamosa leaf was found to exhibit the strongest antioxidant activity in vitro. Column chromatography and thin-layer column chromatography of the ethyl acetate fraction lead to an isolated compound. The structure of the isolated compound was examined using 1-D and 2-D NMR which revealed very intense benzene proton and carbon, an amide alongside a sugar glycone while Gas Chromatography Mass Spectrometer showed significant abundance of a benzolated compound which belong to the aporphine class of alkaloids.
Umar F. Ahmad, Hassan Braimah Yesufu*, Abdulqadir B. Bababe, Boniface Josephus, Garba T. Mohammed.
Objective: The main objective of this study is to show a comparison between Branded and generic Anti-hypertensive and Anti-diabetic medicines, to analyze the beliefs, perceptions, and attitudes of healthcare providers in a tertiary-care hospital toward generic and branded drugs, to analyze the cost between branded and generic drugs of anti-hypertensive and anti-diabetic medicines. Method: A questionnaire-based study was conducted by administering a questionnaire to physicians, pharmacists, and patients registered in Amravati, Talegaon, Arvi, and Katol in the Maharashtra state. Results and Discussion: A comparative study was carried out by the survey on different branded and generic drugs of antidiabetic and antihypertensive category. Conclusion: The survey has shown that there are Negative perceptions about generic medicines and preferential promotion of branded medicines over generics by pharmaceutical companies that could influence prescriber behavior and affect trust in healthcare so as to improve the practice of generic medicines.
Indoles are essential compounds due to their wide biological and pharmacological applications. Here, we describe the synthesis of indoles by intramolecular cyclization of benzylidene hydrazone derivatives following the method of Meth-Cohn and Sushitzky. These compounds were obtained by first designing benzylidene hydrazone derivatives (3a-c) by condensation reaction of N-substituted aldehydes (2a-c) with tosylhydrazine. N-substituted aldehydes (2a-c) were obtained by the action of cyclic amine on 2-chloro-5-nitrobenzaldehyde (1). Finally, sodium methanoate reacts on benzylidene hydrazones (3a-c) under ethanol reflux and led to the indoles (4a-c). The structures of thesecompounds were determined by 1H, 13C Nuclear Magnetic Resonance (NMR) spectroscopy, and High-Resolution Mass Spectrometry (HRMS) analysis. The structure of compound 4b was determined by X-ray crystallography.
This study offers a synthesis of already published analysis methods Cefaclor, either alone or in combination with other medications. Many spectroscopic techniques, including derivative and chromogenic approaches, are available. There is also a novel and improved chromatographic technique based on biological fluids and pharmaceutical formulations. There are a few LC-MS/MS and HPTLC procedures in addition to these two ways. The quality through design or professional method is employedin today's analytical research environment to acquire a better way for method validation. This brief review assignment might help an analyst determine the optimal way for developing and validating analytical procedures.
Banana belongs to Musaceae family and is believed to have originated in Indo-Malaysian region. Banana is rich in carbohydrate, fat, protein, vitamins, minerals, water; not only a part of human diet but it is also used as a feed for domestic animals. Banana is considered as one of the most important commercial crops in the world. The present study has focused on the in vitro antimicrobial, antioxidant and anticancer effect of crude. Musa pulp extract on bacteria (S.aureus, P.aeruginosa, K.pneumoniae and E.coli), antioxidant (DPPH assay and total antioxidant capacity) and anticancer activity against MG63 cell line. Five Musa varieties collected from Kerala, South India were used in this analysis. The fruit of the varieties were extracted with various solvents. Musa accuminata Colla (AA) (Kadali: S4) ethanol extract showed high antimicrobial and antioxidant activity. Bioactive compounds like flavonoids, tannins and polyphenolic compounds were present in the pulp extract. This sample was used in anticancer examination in the MG63 Cell line. Musa fruit extracts expressed a high level of cytotoxic effect on MG 63 bone tumor cell lines with a high scope of usage as an edible drug in cancer treatment.
Anu Augustine, Geethalakshmi Sundararaman*, Prem Jose.