A novel, simple, precise, and accurate reverse phase High Performance Liquid Chromatographic method for estimation of Doxorubicin and Hydroxychavicol was developed and validated according to ICH guidelines. HPLC method was developed using C18, (150mm × 4.6mm, 3.5μm) column with 1% Acetic acid in water: Methanol (45:55 v/v) as a mobile phase at a flow rate of 0.8ml/min and eluents were detected at 284 nm. The calibration curves were linear over the concentration range of 2.5 to 30ng/ml (R2 = 1) for Doxorubicin and 5 to 60ng/ml (R2 = 0.9998) for Hydroxychavicol. The average retention time of Doxorubicin and Hydroxychavicol was 1.17 min and 3.36 min respectively. Average percentage recoveries of Doxorubicin and Hydroxychavicol were 100.15± 0.34 and 100.55± 0.28 %, respectively. The LOQ values for Doxorubicin and Hydroxychavicol were 0.2897 and 0.4197ng/ml respectively. Intra- and inter-day precision values (% RSD) of proposed method were less than 2%.

Communicable diseases are very serious problem at present as well as in past also in developing in addition to developed countries. It is caused by various pathogenic microbes like fungi, bacteria, parasites and virus etc. In recent years, there has been a growing interest in researching and developing new antimicrobial agents from various sources to combat microbial resistance. Therefore, a greater attention has been paid to antimicrobial activity screening and evaluating methods. Herbal medicines are generally used for healthcare because they have low price and wealthy source of antimicrobial properties. Antimicrobial activity of various plant parts such as bark, stem, leaf, fruit and seed against Gram negative, Gram-positive bacteria and fungal species, using hydroalcohol extraction by well diffusion method. The microorganisms employed were Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi and Aspergillus flavus, Aspergillus niger, Aspergillus fumigatus, Penicillium sp. and Aspergillus terreus. Hydroalcoholic extract of crotalaria verrucosa were potentially efficient with variable efficiency against the tested bacterial and fungal strains at different concentrations.

The objective of the present study was to evaluate the phytoconstituents and in vitro hepatoprotective activity of extract of Psidium cattleianum Sabine leaves. The HPTLC profiling of plant extracts were carried out. The in-vitro hepatoprotective activity of hydroalcoholic extract of P. cattleianum (HAEPC) was screened by MTT Assay using Silymarin as standard. The HAEPC showed a significant hepatoprotective activity. The percentage cell viability was identified using MTT assay (In-vitro). The 50 Value of HAEPC was estimated. Results obtained suggests that the hydroalcoholic extract of Psidium cattleianum Sabine leaves exhibits a significant hepatoprotective activity.

The present investigation includes evaluation of an in vitro antidiabetic activity of Psidium cattleianum Sabine leaves extracts. Plant material was subjected to the extract preparation by soxhlet apparatus by using various solvents such as n-hexane, chloroform and 70% ethanol. The different phytochemical constituents present were detected. The antidiabetic activity of the plant were detected by using alpha amylase and alpha glucosidase enzyme in an in vitro model. The result suggests that the hydroalcoholic extract of P. cattleianum leaves exhibits a dose-dependent increase in percentage inhibitory activity on α-glucosidase enzymes (IC50 143.49±10.14 µg/ml) and αamylase (IC50 of 120.2.3µg/ml). Acarbose was used as a standard drug. It is concluded that P. cattleianum leaves exhibits prominent antidiabetic activity.

The objective of the present study is to formulate and evaluate Carica papaya leaves aqueous extract chewable tablets. Carica papaya leaves aqueous extract exhibited potential activity against Dengue fever. Carica papaya leaves are a rich source of complex biochemical constitutes including flavonoids, glycosides, alkaloids, glutathione and glucosinolates that helps boost the number of platelets by stimulating bone marrow and it responsible for production of platelets. The direct compression method was used for the preparation of chewable tablets. Carica papaya chewable tablets were characterized by weight variation, hardness, friability, drug content uniformity, dissolution and short-term stability studies. These tablets are intended to disintegrate smoothly in the mouth at a moderate rate either with or without actual chewing, characteristically chewable tablets have a smooth texture upon disintegration, are pleasant taste. Geriatric and pediatric patients and travelling patients who may not have ready access to water are most need of easy swallowing dosage forms like chewable tablets. In this study the formulation In-vitro drug release studies revealed that the cumulative % drug release of Carica papaya chewable tablets was found to be 86.3%. The bulk density and tapped density of the formulation was found to be 0.588gm/ml and 0.800gm/ml respectively. The result of Carr’s compressibility index and Hausner’s ratio for the formulation shows fair flow properties.

Pain as an unpleasant sensory and emotional experience associated with actual or potential tissue damage. Inflammation is a response to noxious or injurious stimuli, characterized by warmth, redness of the skin, pain, swelling and loss of function. Skeletal muscle relaxants are drugs that are used to relax and reduce muscle tension. They act as both antispasmodic and anti-spasticity agents. Nycthanthes arbortristis flowers consist of essential oils, Nyctanthin, D-mannitol, tannins, flavonoids, glucose, Quercetin, Kaemferol, Apigenin, Anthocyanin, carotenoids, and glycosides and found to have analgesic and skeletal muscle relaxant properties. The main objective of this study is to evaluate the skeletal muscle relaxant activity and analgesic activity of ethanolic extract of the flowers of Nyctanthes arbortristis (ENATF) using Rota-Rod method and hot plate method. High doses of ENATF (500mg/kg) showed highly significant reduction in the time spent by the animals on the revolving rod when compared to the control (P<0.0001). ENATF at all the doses (250mg/kg and 500mg/kg) showed highly significantly (P<0.0001) increased pain latency thermally induced by hot plate method. Thus, the result suggested that the ENATF possess skeletal muscle Relaxant activity and analgesic activity may be due to presence of different chemical compounds present in the extract.

The present research study describes the development and validation of an in-vitro dissolution method for Pramipexole dihydrochloride ER tablets by using reverse phase high performance liquid chromatography technique in different media. The method was developed and validated according to ICH guidelines. Dissolution method was developed in different media of pH 6.8 Phosphate buffer, pH 4.5 buffer and 0.1 NHCl and dissolution profiles up to 24 hrs. In respective dissolution medium were estimated. A simple isocratic analytical method was developed and was found to be accurate, precise, specific and linear. Thus, the proposed dissolution method and analytical method can be applied successfully for the quality control of Pramipexole dihydrochloride in marketed tablets.

In this present study of the Design, Synthesis and molecular docking studies of 2-styrylchromone derivatives as novel antioxidant agents was considered chromone as basic fundamental pharmacophore in order to show antioxidant properties. We have performed molecular docking studies of synthesized compounds to check the ligand interactions with target protein. In addition, ADMET studies and molecular simulations studies also performed to know the toxicity and other parameters of synthesized molecules. Finally, through molecular modelling studies it is concluded that the synthesized molecules follows Lipinski rule of five.

The aim of the present investigation was to formulate Immediate Release Tablets of olanzapine, an anti-psychotic drug. Immediate Release Tablets of Olanzapine were prepared with the addition of different superdisintegrants, namely, crospovidone, croscarmellose sodium, and sodium starch glycolate. Each of these superdisintegrants was used in concentrations of 1% w/w, 2% w/w, 3% w/w. Formulation with 3% w/w crospovidone showed maximum Drug Release (96.44%). The formulation was optimized successfully. The prepared batches were evaluated for organoleptic properties, hardness, friability, weight variation, in vitro drug release studies. The drug-excipient interaction was studied by Fourier transform infrared spectroscopy (FTIR) studies. The optimized formulation showed minimum disintegration time and an almost complete release of the drug within 45 minutes. Finally it was concluded that the Immediate Release Tablets of Olanzapine could be successfully formulated by adding superdisintegrants with improved patient compliance.

Objective: To investigate the anti-atherosclerotic activity of ethanol extract of Polygonum galbrum in male Wistar rats. Material and methods: In that model regarding atherosclerosis, 30 adult male wistar rats were always broken in 5 groups. Group-1 then Group-2 served namely untreated yet model controls respectively, whilst Group-3, four or 5 were the redress agencies which have been simultaneously treated including standard, 200 and four hundred mg/kg eliminate respectively alongside along High Fat Diet. On remaining day, blood samples because biochemical parameters, have been obtained below inhaled diether anaesthesia. Results: HFD induced atherosclerosis as evidenced by marked elevation in cholesterol, Triglycerides, LDL, VLDL and decrease in HDL ranges. Co-management of extract with HFD decreased upward thrust ldl cholesterol, Triglycerides, LDL, VLDL and increase in HDL tiers. Conclusion: It changed into observed that the ethanol extract of Polygonum galbrum conferred Anti- atherosclerotic interest by way of biochemical remark towards HFD triggered atherosclerosis in rats. Within the near destiny could constitute a result in discovery of a singular drug for remedy of drug brought on atherosclerosis.