The objective of the research work is to enhance the solubility and dissolution rate of Nifedipine by converting them into suitable solid lipid nanoparticles (SLNs). The method used for formulating solid lipid nanoparticles (SLNs) was high pressure homogenization followed by solvent emulsification- ultrasonication. The evaluation parameters for SLNs were drug content, entrapment efficiency, particle size, solubility study, in vitro drug release, etc. Nifedipine loaded solid lipid nanoparticles (SLNs) were characterized for drug content, entrapment efficiency, particle size, solubility study and in vitro drug release. Nifedipine loaded SLNs was prepared by using stearic acid and glycerylmonostearate as lipid and Tween 80 as stabilizer. The optimized batch (F4) contained 500mg of Glycerylmonostearate and 300mg of stabilizer. Batch F4 exhibited drug content of 88.75±0.510%, %EE of 79.31±0.119%, Particle size of 146±31.2nm, Practical yield of 91±1.21%, Solubility of 0.011mg/ml and % drug release of 71.17% at 180mins. It was concluded that the solid lipid nanoparticles (SLNs) developed by this method showed increase in solubility and dissolution rate of Nifedipine.
North western region of India, particularly, Rajasthan state is well known for its rich biodiversity. The vast diversity includes not only the cultivated plants but weed plants also. These weed plants are a great source of phytochemicals with medicinal properties. This study focused with four medicinal weed plants including Datura stramonium, Oxalis corniculata, Tridax procumbens, and Phyllanthus niruri. Phytochemical compounds contribute as an important source of nutrition which makes the plants a great source of medicinal values. This research aimed to analyze the chemical compounds including alkaloids, flavonoids, terpenoids, tannins, glycosides, saponins, and carbohydrates which are well known to contain antifungal properties against microorganisms including bacteria and fungi. The research includes the medicinal weed plants from the state of Rajasthan located towards the northwestern region of India. Leaves, stem, and roots of selected medicinal weed plants were processed under aseptic conditions for the plant extraction process using three different solvents including aqueous, ethanol, and methanol. Phytochemicals are known for curing various plant diseases and hence could play an important role in agriculture and crop protection. Our analysis provided evidence that aqueous and other two organic solvents tested with the medicinal plant extracts showed positive results of the presence of important phytochemical constituents which are known to cure several diseases of plants.
Objective: The simple, rapid, reproducible, sensitive and economical method of UV Spectrophotometry for the estimation of Paliperidone (PPD) in bulk and its formulation was developed and validated. Methods: The UV spectrum of Paliperidone in Dimethylformamide (DMF) showed λ max at 280nm. The linearity was established in the concentration range of 10-60µg/ml for Paliperidone. This method was validated for different analytical parameters such as linearity, accuracy, precision, ruggedness and robustness. Results: The method shows approximate linearity over the concentration range of 10-60µg/ml with the regression equation y = 0.0187x - 0.1258 and regression correlation coefficient r2= 0.999 at 280nm. However, the method was found to be highly precise with LOD (1.82) and LOQ (6.07). Conclusion: Considering above results the developed method can be successfully applied for the determination of Paliperidone in different pharmaceutical dosage forms.
Minal P. Khanapure*, Deepak S. Bhosale, Naziya Kalyani, Komal Hotkar.
Objectives: A new, economical, sensitive, simple, rapid UV spectrophotometric method has been developed for the estimation of Benzoyl peroxide in pure form and pharmaceutical formulation. Method: This UV method was developed using methanol as a solvent. In the present method the wavelength selected for analysis was 245nm. UV-Visible double beam spectrophotometer (Systronic 2201) was used to carry out spectral analysis. The ICH guidelines were used to validate the method. Results: The method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. Linearity was found in the range of 5-25µg/ml. Accuracy was performed by using recovery study. The amount of drug recovered was found to be in the range of 100.1-100.5%. The % RSD value was found to be less than 2. Conclusion: The proposed UV spectroscopic method was found to be accurate, precise, stable, linear, specific, and simple for quantitative estimation of benzoyl peroxide in bulk and pharmaceutical dosage form. Hence the present UV spectroscopic method is suitable for routine assay of Benzoyl peroxide in bulk and pharmaceutical formulations.
The simple, rapid, sensitive and specific method of spectrophotometrically was developed for the validation of nifedipine in solid pharmaceutical dosage form i.e. tablet formulation. The UV spectrum of nifedipine in methanol showed λ max at 249nm. The linearity was established in the concentration range of 10-60µg/ml for nifedipine. This method was validated for different analytical parameters such as linearity, accuracy, precision, ruggedness and robustness. The method has been shown approximate linearity over the range of 10-60µg/ml with the regression equation y=0.007x+0.022 and regression correlation coefficient r2= 0.996. However, the method was found to be highly precise with LOD (0.041) and LOQ (0.12). Considering above results the developed method can be successfully performed for the assay of nifedipine in different pharmaceutical dosage form.