Objective: Development of an accurate, simple, precise and rapid method for estimating lamivudine and Tenofovir disoproxil fumarate, simultaneously, in a combined tablet form. Determination of lamivudine and tenofovir disoproxil fumarate were estimated by RP-HPLC using Methanol: Ammonium acetate buffer solution (50:50) as mobile phase at pH 3.5 adjusted ortho phosphoric acid (OPA) with flow rate 1.0ml/min. Column used Kromasil C18 (250mm X 4.6mm i.d.) 5μm as a stationary phase. Result: The retention time were found to be 22 minutes of lamivudine and tenofovir disoproxil fumarate and peak was observed at 260nm which selected wavelength for quantities estimation. The LOD of Lamivudine and Tenofovir disoproxil Fumarate was found to be 0.99µg/ml and 0.58µg/ml. The LOQ of Lamivudine and Tenofovir disoproxil Fumarate was found to be 3.01µg/ml and 1.76µg/ml. Conclusion: The developed RP-HPLC method was simple specific accurate precise and robust for detection of Lamivudine and Tenofovir disoproxil Fumarate in bulk and tablet dosage form.

Introduction:Increased use of antibiotics in hospitals led to multidrug resistant organisms (Klebsiella species). The purpose of this study was to know prevalence of extended spectrum beta - LACTAMASE (ESBL) producing multidrug resistant Klebsiella species from clinical isolate from patients admitted to Dr. Jagdale Mama Hospital and Research centre, Barshi. Dist Solapur (MS) Methodology: A total 310 specimens including urine, pus blood was isolated and identified by standard methodology. An antibiotic susceptibility testing was done by Kirby Bauer disc diffusion method. For detection of extended spectrum beta -lactamases producing isolates combination disk method was done Result: A total of 310 samples (200 urine, 60 pus, and 50 bloods) were included in study. Out of total samples 120(38.8%) samples precede showed growth in e Gram- negative bacteria. Amongst these significant growths 70(58.4%) Klebsiella species, 50(71.4%) Klebsiellapneumoniae and 20(28.5%) Klebsiellaoxytoca were isolated. Amongst these total isolated Kpneumoniae, 35(70%) multidrug resistant Klebsiellapneumoniae and 13(65%) multidrug resistant Klebsiellaoxytoca were isolated. A total of 23(65.7%) Kpneumoniae and 6(46.1%) Koxytoca isolates were found to be extended spectrum beta-lactamase producers.

The present work focusses on the analytical development and validation of ivabradine in bulk and pharmaceutical dosage form by RP-HPLC method. It is a simple, Accurate, precise method, using the C8 column with length of 250mm and 4.5mm diameter, 5mm, contain mobile phase potassium hydrogen phosphate: Acetonitrile 45:55 was pumped through column at a flow rate of 1.0ml/min. At temperature of 30°C. With wave length of 260.0nm. The Retention time of Ivabradine was found to be 2.517min, %RSD found to be 0.6. %Recovery was obtained as 99.85% for ivabradine, by the regression equation the LOD, LOQ, R were 0.33, 0.99 and 0.998, the linearity was found to be y = 17338x + 1317.6 R² = 0.9998. Based on the result the method can useful for routine quality control for analysis of ivabradine.

A simple, precise, accurate, rapid and sensitive HPLC method was developed for simultaneous estimation of Lamivudine and Dolutegravir sodium in tablet formulation. The chromatographic separation was attained on a Waters C18 column (150×4.6mm, particle size 5µ) in isocratic mode using Agilent 1260 Infinity, high performance thin layer chromatography (HPLC) with UV detector. The mobile phase comprised of buffer (pH adjusted to 3.0 using orthophosphoric acid), acetonitrile and methanol (55:35:10 v/v). Water (pH adjusted to 3.3 with orthophosphoric acid) and acetonitrile (58:42 v/v). The flow rate and injection volume were 1.1ml/min and 20µL respectively and the detection was carried out at 260nm using an UV detector. The developed method was validated as per ICH Q2B guidelines for linearity, accuracy, precision, robustness, limit of detection and limit of quantification. The linearity for Lamivudine and Dolutegravir sodium was found to in the range of 18-90µg/ml and 3-15µg/ml respectively with correlation coefficient (r2) > 0.99. The assay of marketed formulation was found to be 99.75% and 99.09% for Lamivudine and Dolutegravir sodium respectively. The recoveries for Lamivudine and Dolutegravir sodium was found to be 99.63% and 99.75% at 80% level, 99.37% and 99.78% at 100% level and 100.15% and 100.47% at 120% level respectively.

A new, simple, precise, accurate and reproducible RP-HPLC for analytical method development and validation of Ribociclib and Palbociclib in bulk form. Separation of Ribociclib and Palbociclib was successfully achieve on Kromasil C18 column with mobile phase containing of 20mM Phosphate buffer (pH-5): Methanol: Acetonitrile (40:30:30, v/v/v). The flow rate was adjusted to 1ml/min with PDA detection at 260nm. With a retention time of 3.47 and 4.48 minutes for Ribociclib and Palbociclib respectively. The method was validated and their response was found to be linear in the drug concentration range of 1µg/ml to5µg/ml for Ribociclib and 5µg/ml to 25µg/ml for and Palbociclib. The values of the correlation coefficient were found to 0.999 for Ledipasvir and 1 for Sofosbuvir respectively. The LOD and LOQ for Ribociclib were found to be 0.088 and 0.267 respectively. The LOD and LOQ for Palbociclib were found to be 0.692 and 2.2097 respectively. This method was found to be good percentage recovery for Ribociclib and Palbociclib were found to be 99.01 and 100.11 respectively indicates that the proposed method is highly accurate. The specificity of the method shows good correlation between retention times of standard with the sample so, the method specifically determines the analyte in the sample without interference from excipients of tablet dosage forms.

With this study, the various pharmacognostic and phytochemical standards for the aerial portions (leaves and stem) of the plant Holostemmaada-kodien were to be established (Asclepiadaceae). The plant Holostemmaada-kodien is traditionally used as an alterative and astringent to the bowels; it also has medicinal properties for ulcers, biliousness, "kapha," blood disorders, worms, itching, leucoderma, and vesicular calculi (Ayurveda). Diabetes, stomachic, gonorrhoea, cough, tonic. To fully harness this folk herb's therapeutic potential, an effort has been made to correctly identify it. According to this perspective, the morphoanatomy of the leaves and stem, along with quantitative microscopy, microscopic linear measurements, WHO-recommended physico-chemical determinations, and genuine phytochemical procedures, are the key diagnostic characters that have been carried out to help the full pharmacognostical evaluation of the plant. The parameters discussed in this research could be suggested as the benchmarks for determining the veracity of Holostemmaada-kodien. This research aids in separating this medication from its other species.