A simple Reverse phase liquid chromatographic method has been developed and subsequently validated for simultaneous determination of Diacerein and Aceclofenac in combination. The separation was carried out using a mobile phase consisting of dipotassium hydrogen phosphate buffer of pH 4, Acetonitrile and methanol in the ratio of 60: 40 % v/v. The column used was Phenomenex Luna C18 Column (150mm× 4.6 mm id 5) with flow rate of 1 ml / min using UV detection at 267 nm. The described method was linear over a concentration range 2-10/ml and 20g/ml of 2for the assay of Diacerein and Aceclofenac respectively. The retention times of Diacerein and Aceclofenac were found to be 0.9927 and 0.88322 respectively. Results of analysis were validated statistically. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Diacerein and Aceclofenac bulk drug and in its pharmaceutical dosage form.

A simple, rapid and reproducible RP-HPLC method was developed and validated for the estimation of Prasugrel Hydrochloride in tablet dosage form. A Zorbax, SB-Phenyl 250x4.6mm, 5µ in isocratic mode, with mobile phase containing Buffer (pH 6.5 with Triethylamine and Potassium dihydrogen ortho phosphate): Acetonitrile: Water (90:10) [40:60 (v/v)] was used. The flow rate was 1.2 ml/min and the analyte was monitored at 235 nm. The retention time for Prasugrel HCl was 11.5 mins. Linearity was obtained in the concentration range of 50ppm to 150ppm with correlation coefficient of 0.999. The percentage recovery of Prasugrel was found to be in the range of 98%-102%.

A simple, rapid, selective and quantitative HPTLC method has been developed for determination of Piperine in Ayurvedic formulations of Chitrak Haritaki of different manufactures. The alcoholic extract of Chitrak Haritaki, Pippali fruit and Kalimirch fruit samples were applied on TLC Aluminium plate pre coated with Silicage l60 GF254 and developed using Toluene Ethyl acetate (9:1) V/Vas mobile phase. The plate was sprayed (derivatized) with Anisaldehyde Sulphuric Acid reagent followed by heating at 110⁰C for 10 minutes and detection and quantification were carried out densitometrically using an UV detector at wave length of 254 nm. Content of marker compound in the samples were found similar.

A new flavonoid compound was isolated from the fruit extract of Acacia nilotica. The compound showed significantly high antioxidant properties in comparison with that of the standard used. The structure suggested as 5, 7, 4^′, 5^′-tetrahydroxy-3-methoxy-flavone (methyl derivative of quercetin) has been elucidated based on the FT-IR, ¹HNMR and ¹³CNMR spectroscopic data analyses.

Antimicrobial and insecticidal activities of different extractives (petroleum ether, chloroform, ethyl acetate, and methanol) as well as three unidentified compounds isolated from the seeds extract of Corchorus capsularis have been investigated. It was observed that all the fractions and the isolated compounds showed antimicrobial and insecticidal activities against four pathogenic bacteria V.cholerae, Shigella spp., Salmonella spp.,E.coli and the insect T. castaneum. The antimicrobial activity test revealed that the crude methanol extracts have the highest antibacterial activity with inhibition zone of 26-27 mm and the isolated compounds showed the activity with zone inhibition of 20-15 mm. In the insecticidal activity test, the petroleum ether fraction showed the highest toxicity than that of the other fractions. The isolated compound also showed similar toxicity against T.castaneum. This study shows the promising potential of Corchorus capsularis in the perspective of the development of new therapeutic agents.