The kinetics of oxidation of thiamine by hexacyanoferrate (abbreviated as HCF) (III) ions in aqueous alkaline medium at constant ionic strength 0.0016 mol/dm3 and temperature 298K has been studied spectrophotometrically. The reaction shows 1:2substrate to oxidant stoichiometry and follows first order kinetics in [alkali]. The dependence of the rate on substrate and [HCF (III)] concentration has been found to be of fractional order type. The ionic strength of the reaction mixture shows positive salt effect on the reaction rate. To calculate thermodynamic parameters the reaction has been studied at six different temperatures from 283K to 333K. Mechanism involving formation of complex between hexacyanoferrate and thiamine has been proposed. 2-[3-[(4-Amino-2-methyl-pyrimidin-5-yl) methyl]-4-methyl-thiazol-5-yl] ethanol have been identified chromatographically and spectroscopically as the final product of oxidation of thiamine. Based on the kinetic data and product analysis a reaction mechanism is proposed.
Ravindra Shimpi, Rajesh Fadat, Bhagwansing Dobhal, D M Janrao, Mazahar Farooqui*.
6 new compounds were isolated for the first time from Trigonellafoenum-graecum seed extract along with one known compound by using simple column chromatographic techniques. They were characterized by chemical and physicochemical evidence and identified as Quercetin 3-O- rutinoside, Chrysoeriol, Monotropein, Di butyl phthalate, De acetyl asperulosidic acid, 1,2 benzene di carboxylic acid and Trigoneoside II a.
The present study was under taken to study the Hepatoprotective activity of ethanol, aqueous and ethyl acetate extracts of Breyniavitis-idaea (burm.f) c. fisher. (Euphorbiaceae) leaves. Hepatoprotective activities of these extracts were studied using in-vitro MTT assay and compared with the toxicants. All the extracts showed significant increase in the % cell viability. Hepatoprotective nature of the extracts may be attributed to the presence of constituents such as alkaloids, glycosides, flavonoids and saponins in plant extracts. This study proven that the potential hepatoprotective nature of the extracts of Breyniavitis-idaea leaves.
