The purpose of this study was to evaluate and compare the anti-inflammatory activity and lipolytic potential of aqueous extract of Rosa Indica (rose), Clitoria Ternatea (butterfly pea) and Cinnamomum Tamala (Indian bay leaf). Anti-inflammatory activity was evaluated usingprotein (egg) denaturation method taking BSA as standard reference. A smartphone application (Photo Metrix PRO app) based on partial least squares regression with the histograms of the red-green-blue images was used to determine the concentration of protein. Lipolytic activity was assessed using milk as dietary substrate and change in acid value post addition of above mentioned extracts in three dilutions (100%, 50%, 10%) were measured titrimetrically. Rosa Indica recorded higher anti-inflammatory activity (at 100cg/ml concentration) followed by Cinnamomum Tamala. Highest acid value was recorded on addition of rose extract followed by butterfly pea and Indian bay leaf in order.
A HPLC method was developed for determination of Related Substances in Levonorgestrel drug substances using Kromasil C18 (250mmx4.6mm, 5mm) column and a mobile phase containing water in gradient combination with acetonitrile (ACN) with flow rate of 1.0mL/min and the chromatogram was monitored using UV Detector at 240nm wavelength. Resolution between Levonorgestrel and its impurities were found more than 1.5 and the stability of test solution and Standard solutions were found upto 24 hours. The LOD and LOQ for Levonorgestrel were observed 0.013% and 0.039%. The analytical method validation of proposed method was performed for various parameters like System suitability, Specificity (interference from Blank and Impurities), Force degradation, LOD and LOQ, Accuracy (Recovery), Linearity, System Precision, Method Precision and Intermediate Precision (Ruggedness) along with Solution stability. The range of method was defined from the data of Precision, Linearity and Accuracy. The Range of method has LOQ to 150% of specification limit. All results for all the validated parameters were found within the acceptance criteria. HPLC method was specific, accurate, precise and suitable for the analysis of Levonorgestreldrug substance and drug products.
A simple and efficient protocol is developed for the synthesis of series of the 2, 3-di-substituted quinazolin-4-(3H)-ones derivatives have been synthesized as a one pot synthesis of the reaction of 2-amino-N-phenyl benzamide and a various substituted aromatic aldehyde in the presence ofLewis acid catalyst asa catalyst in ethanol as a solvent. The final derivatives can be evaluated by 1HNMR, 13CNMR and Mass and in addition of the derivatives evaluated by antimicrobial activity. This method provides several advantages such as high yield, shorter reaction time, mild reaction condition, operational simplicity, easy work-up procedure with environment friendly nature and purification of products by non-chromatographic methods has been developed.
