An isocratic Simultaneous estimation by RP-HPLC Method were developed and validated for the quantification of Cefixime and Ofloxacin in tablet dosage form. Quantification was achieved by using a reversed-phase C18 column (INERTSIL Column, 5µ, 250 mm × 4.6 mm) at ambient temperature with mobile phase consisting of Ammonium acetate Buffer buffer: Acetonitrile: Methanol (50:30:20 pH:6.5)). The flow rate was 1.0 ml/min. Measurements were made at a wavelength of 226nm. The average retention time were found to be 2.39 min for Cefixime and 4.06 min for Ofloxacin. The proposed method was validated for selectivity, precision, linearity and accuracy. The assay methods were found to be linear from 60-140µg/ml for Cefixime and 60-140µg/ml for Ofloxacin. All validation parameters were within the acceptable range. The developed method was successfully applied to estimate the amount of Ofloxacin and Cefexime in tablet dosage form.

Seeds of P. odontadenius were obtained after oven drying at 45°C and they were immersed in SA at concentrations ranging firstly between 0 to 10 mM; secondary between 0 to 20 mM. Seeds were germinated on media and plantlets were transferred in situ. Results showed that SA had positive effects on growth parameters of P. odontadenius in the M1 generations with greater effects observed with treatment exceeding 10 mM. In vitro antimalarial activities from to extracts obtained with aerial materials part from directly immersed seeds (M1), the effects observed with extracts plant from seeds dipped in SA were higher than those from untreated seeds. IC50 values were ranged between 1.04±0.02 μg/ml (10 mM) to 12.77±5.83 μg / ml (0 .26 mM) for the first assay. The second test, the in vitro antiplasmodial activities varied between 1.47±1.07 μg/ml (10 mM) to 21.60±7.13 μg/ml (2.5 mM) for. The best activities were observed with SA solutions exclusive of 5 mM to 10 mM. SA lethal doses were 4.76 mM for LD30 and 10.99 mM for LD50. In vitro antiplasmodial activity on the clinical isolates P. falciparum showed low antimalarial activities from M1 controls (0 Gy) than that of extracts from treated plants. High inhibitory effects (1,04±0.02 μg/mL or 1.47±1.07 μg/mL for 10 mM) of crude extracts plants from treated seeds justified the usefulness of SA in the increasing production of secondary metabolite against malaria in Nigeria.

The addition of synthetic antioxidants to oils and/or foods is one of the most efficient ways to prevent lipid oxidation. However, the safety of synthetic additives has been questioned stimulating the evaluation of naturally occurring compounds with antioxidative properties. Although there is no assurance of the safety of natural antioxidants, there is some comfort knowing that such antioxidants are purified from natural products that have been consumed for generations.Phenolic compounds in plants are recognized as important compounds in conferring stability against oxidation. Natural antioxidant phenolics can be classified into a lipophilic group, tocopherols, and a hydrophilic group, including simple phenolics, phenolic acids, anthocyanins, flavonoids and tannins. Even though chemists have elucidated the structures of thousands of phenolics, there are still many compounds that have not yet been fully characterized and they are referred as phenolic extracts. In this ways berry extracts, aromatic plant extracts, essential oils and their components are gaining interest because of their relatively safe and wide acceptance by consumers. Many authors have reported antioxidant and radical-scavenging properties by berries, spices and essential oils. In this work we reviewed the most important groups of natural antioxidants, with some peculiarities related to chemical composition. Oxidative stress can be caused in result of free radicals formation. Aging and different chronic diseases including diabetes, cancer and cardiovascular diseases could be caused by oxidative stress. Antioxidants are important factor to maintain optimal cellular and human body health. Medicinal plants are an important source of antioxidants16. Natural antioxidants increase the antioxidant capacity of the plasma and reduce the risk of certain diseases. four synthetic antioxidants are widely used in foods; namely, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), propyl gallate (PG), and tert-butylhydroquinone (TBHQ).