Chromatography is a versatile laboratory technique that separates mixtures by exploiting differences in molecular interactions with a stationary phase while dissolved in a mobile phase. It serves both preparative and analytical purposes, with methods like column, ion-exchange, gel-permeation and more, each chosen based on specific separation needs.​ High-Pressure Liquid Chromatography (HPLC) is a powerful analytical tool utilizing pumps to push a sample mixture through a column packed with an adsorbent material. Components separate based on their interaction with the stationary phase.​ RP-HPLC, prominent in pharmaceuticals, employs a non-polar stationary phase and a moderately polar mobile phase, separating molecules via hydrophobic interactions. Retention time correlates with a molecule's hydrophobic surface area.​

Bosentan monohydrate is one of the medicines used to treat pulmonary arterial hypertension, is a dual endothelia receptor antagonist. It prevents pulmonary veins from contracting. Because the traditional route of bosentan monohydrate has a modest onset of effect, it is necessary to improve the onset of action by converting it to FDF. In the present study an attempt was made to formulate solid dispersion of bosentan to enhance the solubility. Further the formulated solid dispersion of bosentan was fabricated as an oral film with the suitable polymer such as HPMC E15 and HPMC K15 by solvent casting method. The findings of thickness, weight uniformity, folding durability, tensile, percentage elongation, drug content uniformity, disintegration duration and in vitro dissolution experiments were found satisfactory. At the end of 5 minutes drug release study, Formulation F5, which included 500mg of HPMC E15, had the highest release rate of 89%.